Rabbit GATOR Complex Sampler Kit Monoclonal Antibody

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Cat. No.: DAB-0012055

Size: 20 µL Size: 100 µL Size: Customer Size

Product Information
Clonality	Monoclonal
Host Species	Rabbit
Product Description	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu730 of human Mios protein. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala211 of human NPRL2 protein. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His356 of human WDR59 protein. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2481 of human mTOR. Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human mTOR protein.
Format	Liquid
Purity	Affinity purity

Target Information
Gene Description	The mTORC1 kinase complex plays a critical role in cell growth regulation. mTORC1 activity is modulated by energy levels, growth factors, and amino acids. Four related GTPases interact with raptor in mTORC1, which is necessary and sufficient for mTORC1 activation in response to amino acid signals. The GAP Activity Towards Rags complex interacts with Rag GTPases and is made up of a pair of protein subcomplexes. The GATOR1 subcomplex includes the proteins DEPDC5, Nprl2 and Nprl3, and is a RagA and RagB GTPase-activating protein that negatively regulates mTORC1 signaling. Conversely, the GATOR2 subcomplex is a positive regulator of mTORC1 signaling.The mammalian target of rapamycin is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481.

Target Information

Gene Description

The mTORC1 kinase complex plays a critical role in cell growth regulation. mTORC1 activity is modulated by energy levels, growth factors, and amino acids. Four related GTPases interact with raptor in mTORC1, which is necessary and sufficient for mTORC1 activation in response to amino acid signals. The GAP Activity Towards Rags complex interacts with Rag GTPases and is made up of a pair of protein subcomplexes. The GATOR1 subcomplex includes the proteins DEPDC5, Nprl2 and Nprl3, and is a RagA and RagB GTPase-activating protein that negatively regulates mTORC1 signaling. Conversely, the GATOR2 subcomplex is a positive regulator of mTORC1 signaling.The mammalian target of rapamycin is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481.

Shipping & Storage
Shipping	Shipped at 4 °C.
Storage Instructions	Store at +4 °C short term (1-2 weeks). Store at -20 °C long term.
Storage Buffer	Constituent: 100% PBS

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