Rabbit Autophagy Vesicle Elongation (LC3 Conjugation) Sampler Kit Monoclonal Antibody

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Cat. No.: DAB-0011835

Size: 20 µL Size: 100 µL Size: Customer Size

Product Information
Clonality	Monoclonal
Host Species	Rabbit
Product Description	Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu44 of human LC3B protein (conserved in LC3A), a synthetic peptide corresponding to residues near the amino terminus of human Atg7 protein, a synthetic peptide corresponding to residues surrounding Gln100 of human Atg4B protein, a synthetic peptide corresponding to residues near the carboxy terminus of human Atg4A protein, a synthetic peptide corresponding to residues surrounding Arg40 of human GABARAP protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of Atg3. Antibodies are purified by protein A and peptide affinity chromatography.
Format	Liquid
Purity	Affinity purity

Target Information
Gene Description	Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer. Autophagy marker Light Chain 3 was originally identified as a subunit of microtubule-associated proteins 1A and 1B and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles. The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy. Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins and four Atg4 homologs. The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. GABAA receptor associated protein is an Atg8 family protein with a key role in autophagy, which was originally discovered as a protein associated with the GABAA receptor regulating receptor trafficking to the plasma membrane. Processing of GABARAP involves cleavage by Atg4 family members followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3.

Target Information

Gene Description

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer. Autophagy marker Light Chain 3 was originally identified as a subunit of microtubule-associated proteins 1A and 1B and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles. The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy. Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins and four Atg4 homologs. The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. GABAA receptor associated protein is an Atg8 family protein with a key role in autophagy, which was originally discovered as a protein associated with the GABAA receptor regulating receptor trafficking to the plasma membrane. Processing of GABARAP involves cleavage by Atg4 family members followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3.

Shipping & Storage
Shipping	Shipped at 4 °C.
Storage Instructions	Store at +4 °C short term (1-2 weeks). Store at -20 °C long term.
Storage Buffer	Constituent: 100% PBS

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