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Cat. No.: DAA-001326
| Product Information | |
|---|---|
| Product Name | Phospho-ULK1 (Ser638) Sandwich ELISA Kit |
| Species | Human; Mouse |
| Description | The Phospho-ULK1 (Ser638) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of ULK1 protein phosphorylated at Ser638. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-ULK1 (Ser638) in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of phospho-ULK1 (Ser638).*Antibodies in this kit are custom formulations specific to kit. |
| Component | Phospho-ULK1 (Ser638) Rabbit mAb Coated Microwells; ULK1 Rabbit Detection mAb; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); Cell Lysis Buffer (10X) |
| Target Information | |
|---|---|
| Target Name | ULK1 |
| UniProt No. | O75385 |
| Gene ID | 8408 |
| Target Description | Two related serine/threonine kinases, UNC-51-like kinase 1 and 2. Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors, such as NGF. Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGAP, and syntenin. Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1. Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy, a catabolic process for the degradation of bulk cytoplasmic contents. It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy.~Phosphorylation of ULK1 by AMPK at Ser555 is critical for starvation-induced autophagy, cell survival under conditions of low nutrients and energy, and mitochondrial homeostasis. |
| Alternative Names | ATG1; hATG1; Unc-51-like kinase 1 |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
Ace Therapeutics has a team of experts in the field of endocrine and metabolic research, aiming to provide innovative preclinical contract research solutions to cope with diabetes and its complications. We provide customized solutions and technical support, enabling the transformation of promising concepts into innovative treatments, thus accelerating the drug development process of diabetes.
