Cat. No.: DAA-001314
| Product Information | |
|---|---|
| Product Name | Phospho-Src (Tyr416) Sandwich ELISA Antibody Pair |
| Species | Human; Dog |
| Description | Cell Signaling Technology's Phospho-Src (Tyr416) Sandwich ELISA Antibody Pair is offered as an economical alternative to our Phospho-Src (Tyr416) Sandwich ELISA KitIt. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The phospho-Src (Tyr416) rabbit capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysates are added followed by a total Src mouse detection antibody and anti-mouse IgG, HRP-linked antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-Src (Tyr416).Antibodies in kit are custom formulations specific to kit. |
| Component | Phospho-Src (Y416) Capture Rabbit mAb (100X); Src Detection Mouse mAb (100X); Anti-mouse IgG, HRP-linked Antibody (1000X) |
| Target Information | |
|---|---|
| Target Name | SRC |
| UniProt No. | P12931 |
| Gene ID | 6714 |
| Target Description | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells. Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active. |
| Alternative Names | Proto-oncogene c-Src; pp60c-src (p60-Src) |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
