Cat. No.: DAA-001290
| Product Information | |
|---|---|
| Product Name | Phospho-S6 Ribosomal Protein (Ser240/244) Sandwich ELISA Kit |
| Species | Human; Mouse |
| Description | Phospho-S6 Ribosomal Protein (Ser240/244) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phosphorylated S6 ribosomal protein at Ser240/244. A Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, phospho-S6 ribosomal protein (Ser240/244) is captured by the coated antibody. Following extensive washing, an S6 Ribosomal Protein Mouse Detection mAb is added to detect the captured phospho-S6 ribosomal protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of S6 ribosomal protein phosphorylated at Ser240/244. |
| Component | Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb Coated Microwells; S6 Ribosomal Protein Mouse Detection mAb; Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated); Detection Antibody Diluent; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); ELISA Sample Diluent; Cell Lysis Buffer (10X) |
| Target Information | |
|---|---|
| Target Name | RPS6 |
| UniProt No. | P62753 |
| Gene ID | 6194 |
| Target Description | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation. Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein. |
| Alternative Names | Phosphoprotein NP33; Small ribosomal subunit protein eS6 |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
