Cat. No.: DAA-001048
| Product Information | |
|---|---|
| Product Name | Phospho-PRAS40 (Thr246) Sandwich ELISA Kit |
| Species | Human; Mouse |
| Description | The Phospho-PRAS40 (Thr246) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of PRAS40 when phosphorylated at Thr246. A PRAS40 rabbit antibody has been coated onto the microwells. After incubation with cell lysates, PRAS40 protein (phosphorylated and nonphospho) is captured by the coated antibody. Following extensive washing, a biotinylated phospho-PRAS40 (Thr246) rabbit detection antibody is added to detect the captured phospho-PRAS40 (Thr246) protein. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of PRAS40 phosphorylated at Thr246.*Antibodies in this kit are custom formulations specific to kit. |
| Component | PRAS40 Rabbit mAb Coated Microwells; Phospho-PRAS40 (Thr246) (C77D7) Rabbit Detection mAb (Biotinylated); HRP-Linked Streptavidin (ELISA Formulated); Detection Antibody Diluent; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); ELISA Sample Diluent; Cell Lysis Buffer (10X) |
| Target Information | |
|---|---|
| Target Name | AKT1S1 |
| UniProt No. | Q96B36 |
| Gene ID | 84335 |
| Target Description | Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity. PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183. |
| Alternative Names | 40 kDa proline-rich AKT substrate |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
