Cat. No.: DAA-001244
| Product Information | |
|---|---|
| Product Name | Phospho-MARCKS (Ser152/156) Sandwich ELISA Kit |
| Species | Human |
| Description | The Phospho-MARCKS (Ser152/156) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of MARCKS when phosphorylated at Serines 152 and 156. A MARCKS rabbit antibody has been coated onto the microwells. After incubation with cell lysates, MARCKS protein (phosphorylated and nonphospho) is captured by the coated antibody. Following extensive washing, a biotinylated phospho-MARCKS (Ser152/156) rabbit monoclonal detection antibody is added to detect the captured phospho-MARCKS protein. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of MARCKS phosphorylated at Serines 152 and 156. |
| Component | MARCKS Rabbit mAb Coated Microwells; Phospho-MARCKS (Ser152/156) Rabbit Detection Antibody (Biotinylated); HRP-Linked Streptavidin (ELISA Formulated); Detection Antibody Diluent; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Sample Diluent; ELISA Wash Buffer (20X); Sandwich ELISA Lysis Buffer (1X) |
| Target Information | |
|---|---|
| Target Name | MARCKS |
| UniProt No. | P29966 |
| Gene ID | 4082 |
| Target Description | Myristoylated Alanine-Rich C-Kinase Substrate. Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm. |
| Alternative Names | MARCKS; Protein kinase C substrate, 80 kDa protein, light chain (80K-L protein; PKCSL) |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
