Cat. No.: DAA-001227
| Product Information | |
|---|---|
| Product Name | Phospho-IRS-1 (Ser307) Sandwich ELISA Kit |
| Species | Human; Mouse |
| Description | The Phospho-IRS-1 (Ser307) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of IRS-1 when phosphorylated at Ser307. An IRS-1 Mouse Antibody has been coated onto the microwells. After incubation with cell lysates, IRS-1 (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Phospho-IRS-1 (Ser307) Rabbit Detection Antibody is added to detect phosphorylation of Ser307 on the captured IRS-1 protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-1 phosphorylated at Ser307.*Antibodies in this kit are custom formulations specific to kit. |
| Component | IRS-1 Mouse mAb Coated Microwells; Phospho-IRS-1 (Ser307) Rabbit Detection Antibody; Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated); Detection Antibody Diluent; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); ELISA Sample Diluent; Cell Lysis Buffer (10X) |
| Target Information | |
|---|---|
| Target Name | IRS1 |
| UniProt No. | P35568 |
| Gene ID | 3667 |
| Target Description | Insulin receptor substrate 1. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity. |
| Alternative Names | HIRS 1 antibody; HIRS1 antibody; Insulin receptor substrate 1 antibody; ; ; IRS 1 antibody; IRS-1 antibody; IRS1 antibody; IRS1_HUMAN antibody; OTTHUMP00000164234 antibody; ; see all |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
