Cat. No.: DAA-001219
| Product Information | |
|---|---|
| Product Name | Phospho-Insulin Receptor β (Tyr1146) Sandwich ELISA Kit |
| Species | Human |
| Description | Phospho-Insulin Receptor β (Tyr1146) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects transfected phospho-insulin receptor (Tyr1146) protein. A Phospho-Insulin Receptor (Tyr1146) Antibody has been coated onto the microwells. After incubation with cell lysates, phospho-insulin receptor (Tyr1146) proteins are captured by the coated antibody. Following extensive washing, Insulin Receptor β Mouse mAb is added to detect the captured phospho-insulin receptor (Tyr1146) protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of phospho-insulin receptor (Tyr1146) protein.Antibodies in kit are custom formulations specific to kit. |
| Component | Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Rabbit mAb Coated Microwells; Insulin Receptor ß Mouse Detection mAb; Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated); Detection Antibody Diluent; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); ELISA Sample Diluent; Cell Lysis Buffer (10X) |
| Target Information | |
|---|---|
| Target Name | INSR |
| UniProt No. | P06213 |
| Gene ID | 3643 |
| Target Description | Type I insulin-like growth factor receptor. Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites. Phosphorylation of these three tyrosine residues is necessary for kinase activation. Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation. |
| Alternative Names | CD220; HHF5; human insulin receptor; Insr; INSR_HUMAN; Insulin receptor subunitβ; IR; IR 1; IR-1; IR1 |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
