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Cat. No.: DAA-001218
| Product Information | |
|---|---|
| Product Name | Phospho-Insulin Receptor β (Tyr1146) Sandwich ELISA Antibody Pair |
| Species | Human |
| Description | Phospho-Insulin Receptor β (Tyr1146) Sandwich ELISA Antibody Pair is offered as an economical alternative to our Phospho-Insulin Receptor β (Tyr1146) Sandwich ELISA KitIt. Capture and detection antibodies (100X stocks) and HRP-linked secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The phospho-insulin receptor β (Tyr1146) capture antibody is coated on a 96 well microplate in PBS overnight. After blocking, cell lysates are added followed by an insulin receptor β detection antibody and anti-mouse IgG, HRP-linked antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-insulin receptor β (Tyr1146) protein.Antibodies in kit are custom formulations specific to kit. |
| Component | Phospho-Insulin Receptor β (Tyr1146) Rabbit Capture Antibody (100X); Insulin Receptor β Detection Mouse mAb (100X); Anti-mouse IgG, HRP-linked Antibody (1000X) |
| Target Information | |
|---|---|
| Target Name | INSR |
| UniProt No. | P06213 |
| Gene ID | 3643 |
| Target Description | Type I insulin-like growth factor receptor. Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites. Phosphorylation of these three tyrosine residues is necessary for kinase activation. Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation. |
| Alternative Names | CD220; HHF5; human insulin receptor; Insr; INSR_HUMAN; Insulin receptor subunitβ; IR; IR 1; IR-1; IR1 |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
Ace Therapeutics has a team of experts in the field of endocrine and metabolic research, aiming to provide innovative preclinical contract research solutions to cope with diabetes and its complications. We provide customized solutions and technical support, enabling the transformation of promising concepts into innovative treatments, thus accelerating the drug development process of diabetes.
