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Cat. No.: DAA-001115
| Product Information | |
|---|---|
| Product Name | Phospho-GSK-3α (Ser21) ELISA Kit |
| Species | Human; Mouse; Rat; Monkey |
| Description | Phospho-GSK-3α (Ser21) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of GSK-3α when phosphorylated at Ser21. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-GSK-3α (Ser21) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-GSK-3α (Ser21). |
| Component | ELISA Microwell Strip Plate, 96 Well; GSK-3α Rabbit Capture mAb; Phospho-GSK-3α (Ser21) Rabbit HRP-linked mAb; ELISA Capture Antibody Diluent; ELISA HRP Antibody Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); ELISA Cell Extraction Buffer (5X); ELISA Cell Extraction Enhancer Solution (50X); ELISA KitIt5 Positive Control Type 1 |
| Target Information | |
|---|---|
| Target Name | GSK3A |
| UniProt No. | P49840 |
| Gene ID | 2931 |
| Target Description | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin. GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β. GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development. GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization. |
| Alternative Names | GSK-3α |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
Ace Therapeutics has a team of experts in the field of endocrine and metabolic research, aiming to provide innovative preclinical contract research solutions to cope with diabetes and its complications. We provide customized solutions and technical support, enabling the transformation of promising concepts into innovative treatments, thus accelerating the drug development process of diabetes.
