Cat. No.: DAA-001115
| Product Information | |
|---|---|
| Product Name | Phospho-GSK-3α (Ser21) ELISA Kit |
| Species | Human; Mouse; Rat; Monkey |
| Description | Phospho-GSK-3α (Ser21) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of GSK-3α when phosphorylated at Ser21. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-GSK-3α (Ser21) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-GSK-3α (Ser21). |
| Component | ELISA Microwell Strip Plate, 96 Well; GSK-3α Rabbit Capture mAb; Phospho-GSK-3α (Ser21) Rabbit HRP-linked mAb; ELISA Capture Antibody Diluent; ELISA HRP Antibody Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); ELISA Cell Extraction Buffer (5X); ELISA Cell Extraction Enhancer Solution (50X); ELISA KitIt5 Positive Control Type 1 |
| Target Information | |
|---|---|
| Target Name | GSK3A |
| UniProt No. | P49840 |
| Gene ID | 2931 |
| Target Description | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin. GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β. GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development. GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization. |
| Alternative Names | GSK-3α |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
