Cat. No.: DAA-001089
| Product Information | |
|---|---|
| Product Name | Phospho-eIF4EBP1 (T36) ELISA Kit |
| Species | Human |
| Description | Phospho-eIF4EBP1 (T36) ELISA Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated 4EBP1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-eIF4EBP1. An anti-pan eIF4EBP1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and eIF4EBP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-eIF4EBP1 (Thr36) antibody is used to detect phosphorylated eIF4EBP1. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of eIF4EBP1 (Thr36) bound. |
| Sample Types | Cell Lysate |
| Target Information | |
|---|---|
| Target Name | EIF4EBP1 |
| UniProt No. | Q13541 |
| Gene ID | 1978 |
| Target Description | Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. |
| Alternative Names | 4E-BP1; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1 |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
