Cat. No.: DAA-001088
| Product Information | |
|---|---|
| Product Name | Phospho-eIF4EBP1 (T36) and Total eIF4EBP1 ELISA Kit |
| Species | Human |
| Description | Phospho-eIF4EBP1 (T36) and Total eIF4EBP1 ELISA Kit is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated eIF4EBP1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-eIF4EBP1 and total eIF4EBP1. An anti pan eIF4EBP1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and eIF4EBP1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho eIF4EBP1 (Thr36) antibody is added to detect phosphorylated eIF4EBP1. In the remaining wells, biotinylated anti-pan-eIF4EBP1 antibody is used to detect pan eIF4EBP1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of eIF4EBP1 (Thr36) or pan eIF4EBP1 bound. |
| Sample Types | Cell Lysate |
| Target Information | |
|---|---|
| Target Name | EIF4EBP1 |
| UniProt No. | Q13541 |
| Gene ID | 1978 |
| Target Description | Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. |
| Alternative Names | 4E-BP1; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1 |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
