Cat. No.: DAA-001070
| Product Information | |
|---|---|
| Product Name | Phospho-Beclin-1 (Ser30) Sandwich ELISA Kit |
| Species | Human |
| Description | The Phospho-Beclin-1 (Ser30) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Beclin-1 protein phosphorylated at Ser30. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-Beclin-1 (Ser30) in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of phospho-Beclin-1 (Ser30). |
| Component | Beclin-1 Rabbit mAb Coated Microwells; Phospho-Beclin-1 (Ser30) Rabbit Detection mAb; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); Cell Lysis Buffer (10X) |
| Target Information | |
|---|---|
| Target Name | BECN1 |
| UniProt No. | Q14457 |
| Gene ID | 8678 |
| Target Description | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions. One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30. Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells. Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak. While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers. Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export. Investigators have demonstrated that Beclin-1-/- mice die early in embryogenesis and Beclin-1-/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal. Researchers have also found that overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis.ULK1 phosphorylates Beclin-1 at Ser30 to induce autophagy in response to amino acid starvation, mTORC1 inhibition, and hypoxia. |
| Alternative Names | Beclin-1-C 35 kDa; Beclin-1-C 37 kDa |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
