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Cat. No.: DAA-001063
| Product Information | |
|---|---|
| Product Name | Phospho-Atg14 (Ser29) Sandwich ELISA Kit |
| Species | Human |
| Description | The Phospho-Atg14 (Ser29) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Atg14 protein phosphorylated at Ser29. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with Atg14 protein phosphorylated at Ser29 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of Atg14 protein phosphorylated at Ser29. |
| Component | Atg14 Rabbit mAb Coated Microwells; Phospho-Atg14 (Ser29) Rabbit Detection mAb; HRP Diluent; TMB Substrate; STOP Solution; Sealing Tape; ELISA Wash Buffer (20X); Cell Lysis Buffer (10X) |
| Target Information | |
|---|---|
| Target Name | ATG14 |
| UniProt No. | Q6ZNE5 |
| Gene ID | 22863 |
| Target Description | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related. Atg14 and Rubicon were identified based on their ability to bind to Beclin-1 and participate in unique complexes with opposing functions. Rubicon, which localizes to the endosome and lysosome, inhibits Vps34 lipid kinase activity; knockdown of Rubicon enhances autophagy and endocytic trafficking. In contrast, Atg14 localizes to autophagosomes, isolation membranes and ER, and can enhance Vps34 activity. Knockdown of Atg14 inhibits starvation-induced autophagy.The serine/threonine kinase ULK1 phosphorylates Atg14 at Ser29 to promote autophagosome formation. |
| Alternative Names | Autophagy-related protein 14-like protein (Atg14L); Barkor |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
Ace Therapeutics has a team of experts in the field of endocrine and metabolic research, aiming to provide innovative preclinical contract research solutions to cope with diabetes and its complications. We provide customized solutions and technical support, enabling the transformation of promising concepts into innovative treatments, thus accelerating the drug development process of diabetes.
