Insulin resistance, a fundamental hallmark of metabolic disorders such as type 2 diabetes (T2D), has long been a significant challenge in the field of endocrinology and drug development. At Ace Therapeutics, we are dedicated to developing high-quality in vitro insulin resistance models that enable researchers to explore new frontiers in diabetes research.

Introduction to Insulin Resistance

Insulin resistance is a major factor in the development of diabetes and is closely linked to obesity and metabolic syndrome. It affects insulin-sensitive tissues like adipose tissue, skeletal muscle, and the liver, leading to free fatty acid (FFA) accumulation, increased liver glucose production, mitochondrial defects, and excessive ROS production.

Animal models have traditionally been used in diabetes research, but they have limitations in terms of cost, time, and ethical concerns. In vitro models provide a cost-effective and time-efficient alternative method that allows for precise control of experimental conditions and can replicate the cellular and molecular mechanisms of insulin resistance. Cell-level research using in vitro models has been proven valuable in understanding the pathogenesis of diabetes and insulin resistance and aiding in the discovery of insulin sensitizer candidates.

Fig. 1. Setting up four diverse in vitro insulin-resistance models in 3T3-L1. (Green A D, et al., 2018)

Our In Vitro Insulin Resistance Models

Through rigorous scientific investigation and the integration of state-of-the-art technologies, we can create a comprehensive suite of in vitro models to recapitulate the key hallmarks of diabetes. By leveraging a diverse array of cell types, including adipocytes, hepatocytes, and myocytes, our models faithfully capture the tissue-specific responses and interactions that contribute to the development and progression of insulin resistance.

• Hepatocyte Insulin Resistance Model
  The hepatocyte insulin resistance models we provide are usually derived from animal primary liver cells and the HepG2 cell line. These models can be used to study the pathogenesis of diabetes, enzyme induction, hypoglycemic mechanisms of drugs, and drug efficacy.
• Adipocyte Insulin Resistance Model
  We develop in vitro insulin resistance models using primary human and animal adipocytes and a well-established preadipose cell line 3T3-L1. These models accurately recapitulate the cellular and molecular features of insulin resistance, enabling researchers to study the mechanisms of impaired insulin signaling, dysregulated lipid metabolism, and secretion of proinflammatory cytokines.
• Myocyte Insulin Resistance Model
  Our model, featuring primary human myocytes or myoblasts, recapitulates the key hallmarks of insulin resistance, such as diminished glucose uptake and impaired mitochondrial function. We use these models to help clients understand glucose metabolism, insulin signaling mechanisms, insulin resistance, and oxidative stress at the cellular and molecular levels.

Our Analysis Services for Insulin Resistance Research

Equipped with advanced analytical techniques, we utilize in vitro insulin resistance models to provide a comprehensive view of the metabolic and signaling alterations that characterize diabetes and insulin resistance. Our analysis services include:

By collaborating with Ace Therapeutics, researchers gain access to high-quality in vitro insulin resistance models and comprehensive research services, propelling diabetes research forward. Contact us for additional information about how our models and services can be customized to meet your exact needs.

References

1. Lo K A, et al. (2013) Analysis of in vitro insulin-resistance models and their physiological relevance to in vivo diet-induced adipose insulin resistance. Cell Reports. 5(1): 259-270.
2. Yudhani R D, et al. (2023) In vitro insulin resistance model: a recent update. Journal of Obesity. 2023(1): 1964732.

• Pancreatic Islet Cell Models
• Induction Methods of In Vitro Insulin Resistance Model

• Mouse Models
• Assay Kits
• Antibodies
• Cell Lines & Lysates
• Proteins & Peptides
• Inhibitors & Activators