Chronic insulin exposure is known to be associated with insulin resistance. Ace Therapeutics utilizes chronic insulin exposure to induce in vitro insulin resistance models. Our efficient service process and reliable in vitro insulin resistance models are designed to help our clients achieve breakthroughs in researching diabetes mechanisms, screening drugs, and developing innovative therapies.

Fig. 1 Chronic insulin treatment impairs insulin signalling in both 3T3-L1 and SGBS, causing insulin resistance. (Rossi, A.; et al., 2020)

Our Insulin-Induced In Vitro Modeling Service

• Chronic insulin exposure: We select appropriate cell lines and treat them according to the client's requirements. Chronic hyperinsulinic environments are mimicked by exposing cells to specific concentrations and durations of insulin, thereby inducing insulin resistance in the cells.
• Model validation: We validate the insulin resistance status of in vitro cellular models through comprehensive analytical assays. These assays typically include detection of cellular response to insulin, glucose uptake and utilization, and other biochemical indices, thus ensuring the stability and reliability of the model.
• Antidiabetic drugs screening and assessment: Our in vitro insulin resistance models, induced by chronic insulin stimulation, assist our clients in screening antidiabetic drugs and conducting efficacy evaluations, helping them to accelerate the discovery of promising antidiabetic drugs.
• Data analysis and report: We offer comprehensive data analysis and report interpretation services to provide valuable reference points for subsequent experiments.

Applications of Chronic Insulin Induced In Vitro Models

• Mechanistic studies of diabetes and related metabolic diseases.
• In vitro screening and efficacy assessment of potential antidiabetic drugs.
• Efficacy assessment of food or nutritional supplements.

Advantages of Our Insulin-Induced In Vitro Models

• Customized in vitro model construction services, including optimized cell culture conditions, insulin concentrations and exposure times.
• The reliability of our in vitro model is evaluated through rigorous quality control and comprehensive assessment of insulin resistance status using multiple biomarker analyses.
• Our insulin-induced in vitro insulin resistance models are highly reproducible and provide reliability and accuracy to our clients' research.

Ace Therapeutics is dedicated to leveraging our expertise to develop highly controllable and reproducible in vitro models of insulin resistance for organizations engaged in diabetes research and therapy development worldwide. Please contact us for more details and we will be happy to assist you.

Reference

1. Rossi, A.; et al. In vitro characterization of the effects of chronic insulin stimulation in mouse 3T3-L1 and human SGBS adipocytes. Adipocyte. 9.1 (2020): 415-426.

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