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Cat. No.: DCL-001228
| Product Information | |
|---|---|
| Organism | Rat |
| Tissue | Pancreas |
| Cell Type | Epithelial-like cells |
| Karyotype | Not specified |
| Product Description | RIN-5F is a secondary clone of the rat islet tumour cell line RIN-m. Cloning was performed by limited dilution in RPMI 1640, 10%FBS and 20% conditioned medium. The conditioned medium contained growth hormones and prolactin being produced by rat pituitary tumour cells GH3. RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, L-dopa-decarboxylase and contain membrane-bound secretory granules. |
| Format | Frozen |
| Growth Properties | Adherent |
| Shipping & Storage | |
|---|---|
| Shipping | Cryopreserved on dry ice. |
| Storage Instructions | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Cells grow in small islands and do not form a confluent monolayer. |
| Storage Buffer/Media | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
Ace Therapeutics has a team of wellknown experts in the field of endocrine and metabolic research, aiming to provide innovative preclinical contract research solutions to cope with diabetes and its complications. We provide customized solutions and technical support, enabling the transformation of promising concepts into innovative treatments, thus accelerating the drug development process of diabetes.
