Cat. No.: DAA-001091
| Product Information | |
|---|---|
| Product Name | Human KAT3B / p300 ELISA Kit |
| Species | Human |
| Description | Human KAT3B ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human KAT3B protein in human cell culture samples. It uses our proprietary ELISA technology. Quantitate Human KAT3B with 51 pg/mL sensitivity ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. |
| Sample Types | Cell Culture Extracts, Cell Lysate |
| Detection Range | 94 pg/mL - 6000 pg/mL |
| Sensitivity | 51 pg/mL |
| Target Information | |
|---|---|
| Target Name | EP300 |
| UniProt No. | Q09472 |
| Gene ID | 2033 |
| Target Description | Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122'. Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity. Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity. Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair. Acetylates MEF2D. |
| Alternative Names | E1A associated protein p300; E1A binding protein p300; E1A-associated protein p300; EP300; EP300: E1A binding protein p300; EP300_HUMAN; Histone acetyltransferase p300; KAT3B; p300 HAT; RSTS2 |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at +4°C. Please refer to protocols. |
