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Cat. No.: DAA-001155
| Product Information | |
|---|---|
| Product Name | Human IL-1β ELISA Kit |
| Species | Human |
| Description | Human IL-1β in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1β concentrations in cell culture supernatants, serum and plasma. An IL-1β specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-1β specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-1β captured in plate. The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles. |
| Sample Types | Cell Culture Supernatant, Serum, Plasma |
| Detection Range | 3.91 pg/mL - 250 pg/mL |
| Sensitivity | 1.5 pg/mL |
| Target Information | |
|---|---|
| Target Name | IL1B |
| UniProt No. | P01584 |
| Gene ID | 3553 |
| Target Description | Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. |
| Alternative Names | Catabolin; H1; IFNβ inducing factor; IL 1; IL 1β; IL-1β; IL1; IL1β; IL1B; IL1B_HUMAN; IL1F2; Interleukin 1β; Interleukin 1β precursor; interleukin 1, β; Interleukin-1β; OAF; Osteoclast activating factor; OTTHUMP00000162031; Preinterleukin 1β; Preinterleukinβ; Pro interleukin 1β |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
Ace Therapeutics has a team of wellknown experts in the field of endocrine and metabolic research, aiming to provide innovative preclinical contract research solutions to cope with diabetes and its complications. We provide customized solutions and technical support, enabling the transformation of promising concepts into innovative treatments, thus accelerating the drug development process of diabetes.
