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Cat. No.: DCL-001224
| Product Information | |
|---|---|
| Organism | Human |
| Tissue | Pancreatic islets |
| Cell Type | Epithelial cells |
| Karyotype | Modal chromosome number 67-72 |
| Product Description | 1.4E7 is a hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line. 1.4E7 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology. 1.4E7 cells provide a method of producing pure insulin secreting cells when stimulated (please see attached protocols for the stimulation of insulin secretion). This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis. |
| Format | Frozen |
| Shipping & Storage | |
|---|---|
| Shipping | Cryopreserved on dry ice. |
| Storage Instructions | Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected. |
| Storage Buffer/Media | RPMI-1640 + 2mM Glutamine + 10% FCS |
Ace Therapeutics has a team of wellknown experts in the field of endocrine and metabolic research, aiming to provide innovative preclinical contract research solutions to cope with diabetes and its complications. We provide customized solutions and technical support, enabling the transformation of promising concepts into innovative treatments, thus accelerating the drug development process of diabetes.
