Ace Therapeutics uses high glucose exposure to induce in vitro insulin resistance models to help clients with diabetes research and related drug development. Our extensive experience and efficient service will provide scientifically reliable support for your research.

Overview of High Glucose-Induced In Vitro Models

High glucose concentration may cause insulin resistance in various tissues, including skeletal muscle, liver, and adipocytes. Glucose toxicity caused by chronic high glucose exposure may provoke irreversible damage in pancreatic β cells and promote further development of insulin resistance and secretory dysfunction. Therefore, high glucose concentration can be used as an inducer in models of insulin resistance.

Fig. 1 High glucose induced insulin resistant and ROS over production in HepG2 cells. (Ding, X. et al., 2019)

High Glucose-Induced In Vitro Model Development Services

Ace Therapeutics offers one-stop services coverage from in vitro model construction and model verification to data analysis. We are committed to providing high-quality services to ensure the delivery of reliable in vitro models to our clients.

• Cell culture and processing: Our experienced experts will select the appropriate cell line based on customer needs and culture it in high-glucose medium. The high glucose concentration and exposure time are precisely controlled to ensure reliable in vitro insulin resistance modeling.
• Model validation: We assess the insulin resistance status of the model through a series of analytical experiments to detect cellular response to insulin. These experiments typically assess glucose uptake, phosphorylation levels of insulin receptor substrates (IRS), and activation of downstream signaling pathways, such as the PI3K/Akt pathway.
• Antidiabetic drug screening and evaluation: We help our clients accelerate their antidiabetic drug development programs by providing antidiabetic drug screening and evaluation services through customized in vitro models.
• Data analysis and report: We provide professional services involving experimental data analysis and explanation of reports.

Advantages of Our High Glucose-Induced In Vitro Model Development Services

• Customized services: According to clients' requirements, we offer customized model development service, including cell line selection, determination of glucose concentration and exposure time.
• Standardized procedures: Standardized experimental procedures to ensure data reliability and reproducibility.
• Rapid response: Our efficient service processes help you accelerate diabetes-related research processes.

Applications of High Glucose-Induced In Vitro Models

• Research on the mechanism of action of diabetes mellitus.
• Insulin signaling pathway and cellular metabolism research.
• Screening and evaluation of antidiabetic drugs.

At Ace Therapeutics, we are committed to providing you efficient, stable in vitro insulin resistance model development services that support your diabetes research. Please contact us for more details and we will be happy to assist you.

Reference

1. Ding, X.; et al. Ellagic acid ameliorates oxidative stress and insulin resistance in high glucose-treated HepG2 cells via miR-223/keap1-Nrf2 pathway. Biomedicine & Pharmacotherapy. 110(2019): 85-94.

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• In Vitro Insulin Resistance Models
• Induction Methods of In Vitro Insulin Resistance Model
• In Vitro Insulin Resistance Models Induced by Chronic Insulin Stimulation

• Mouse Models
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• Antibodies
• Cell Lines & Lysates
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• Inhibitors & Activators