Cat. No.: DAA-001103
| Product Information | |
|---|---|
| Product Name | Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit |
| Description | The Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit contains the necessary reagents for rapid, sensitive, and simple detection of G6PD activity in various samples. In the assay, glucose-6-phosphate (G6P), in the presence of NADP, is oxidized by G6PD to generate 6-phosphogluconolactone and NADPH. The generated NADPH is then amplified by the diaphorase-cycling system to produce highly fluorescent resorufin molecules (see Figure 1). The relative fluorescent units (RFU) can then be determined using a plate reader with excitation about 540 nm and emission about 590 nm. The magnitude of RFU is proportional to G6PD activity in the sample. |
| Component | Tris Assay Buffer; G6PDH Substrate (40X); G6PDH Cofactor (100X); NADP+ (100X); G6PDH Developer (100X); G6PDH Positive Control (100X); Sandwich ELISA Lysis Buffer (1X) |
| Target Information | |
|---|---|
| Target Name | G6PD |
| UniProt No. | P11413 |
| Gene ID | 2539 |
| Target Description | Glucose-6-phosphate dehydrogenase (G6PD) catalyses the first, and rate-limiting, step of the pentose phosphate pathway. The NADPH generated from this reaction is essential to protect cells from oxidative stress. Research studies have shown that p53 interacts with G6PD and inhibits its activity, therefore suppressing glucose consumption through the pentose phosphate pathway. In cancer cells with p53 mutations, the increased glucose consumption is directed towards increased biosynthesis, which is critical for cancer cell proliferation. |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
