By manipulating genes involved in glucose metabolism and insulin signaling, researchers at Ace Therapeutics have been able to develop animal models that closely mimic human diabetes. These models provide valuable insights into the molecular mechanisms underlying the pathogenesis of diabetes, allowing for the evaluation of potential therapies.

Overview of Genetically Engineered Diabetes Models

The use of molecular biology techniques has propelled the creation of genetically modified animal models, including knockout and transgenic animals, providing invaluable tools for investigating gene functions, identifying disease mechanisms, and evaluating potential drugs. Traditionally, rodent models of diabetes have been used to achieve these goals. Additionally, to bridge the gap between mouse models and human patients, researchers have used pigs as another classic animal model for diabetes research. With continued advances in genetic engineering, researchers have developed diabetic pig models that mimic human disease mechanisms at the molecular level.

Fig. 1. Potential applications of genetically tailored pigs for diabetes research and therapy. (Wolf E, et al., 2014)

Our Methods for Developing Genetically Engineered Diabetes Models

At Ace Therapeutics, we use a variety of effective and precise gene modification techniques to upregulate or downregulate gene expression through targeted knockouts, knock-ins, and substitutions of specific DNA fragments to develop diabetes animal models with relevant genotypes.

• Lentiviral transgenesis
• Inducible transgene expression
• Site-directed nuclease (SDN) genome editing
• CRISPR/Cas9-mediated gene editing

Our Genetically Engineered Diabetes Animal Models

We offer a variety of animal models to meet your needs.

• Ins2-C96Y Mice
  We provide Ins2-C96Y point mutant mouse models that exhibit obvious diabetic symptoms such as polyphagia, polydipsia, hyperglycemia, and weight loss, which can be used as animal models for type I diabetes research.
• Pancreas-Specific PRLR Knockout Mice
  We construct genetically manipulated models of gestational diabetes mellitus (GDM) by knocking out the prolactin receptor (PRLR) gene, which is a key molecule in maintaining glucose homeostasis in vivo during pregnancy.
• GK/IRS-1 Double Knockout Mice
  We provide GK/IRS-1 double knockout mice that display symptoms of type II diabetes, including insulin resistance and impaired glucose tolerance.
• Other Knockout Mouse Models
  We provide a variety of single-gene knockout diabetes animal models, such as Gck knockout, Hnf4a knockout, and Abcc8 knockout mice.
• Humanized GLP-1R Mice
  We construct animal models for direct screening of diabetes drugs and initial assessment of pharmacological impacts by introducing the human GLP-1R gene into mice.
• Genetically Tailored Pig Models
  We also help customers develop and validate various genetically tailored pig models for diabetes research, such as GHR deficient pigs.

For more information about our diabetes animal model research and development services, please contact us. Ace Therapeutics is here to assist you with all your diabetes research needs.

Reference

1. Wolf E, et al. (2014) Genetically engineered pig models for diabetes research. Transgenic Research. 23: 27-38.

• Spontaneous Models of Diabetes Mellitus
• Diet-Induced Diabetes Models
• Surgically Induced Diabetic Models
• Chemically Induced Diabetes Models

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