Cyclic GMP Chemiluminescent Assay Kit

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Cat. No.: DAA-001078

Size: 1 x 96 tests Size: 10 x 96 tests Size: Customer Size

Product Information
Product Name	Cyclic GMP Chemiluminescent Assay Kit
Description	The Cyclic GMP Chemiluminescent Assay Kit is a competition enzyme-linked immunoassay used to determine cGMP levels in cells or tissues of interest. In this assay, cGMP found in the test sample competes with a fixed amount of HRP-linked cGMP for binding to an anti-cGMP Rabbit mAb immobilized onto a 96-well plate. Following washing to remove excess sample cGMP and HRP-linked cGMP, chemiluminescent reagent is added for signal development. Because of the competitive nature of this assay, the magnitude of light emission, measured in relative light units (RLU), is inversely proportional to the quantity of sample cGMP. Measurement of light emmision using the cGMP Standard allows calculating the absolute amount of cGMP in a sample of interest.
Component	cGMP Rabbit mAb Coated Microwells; cGMP-HRP Conjugate; cGMP Standard (5 uM); Luminol/Enhancer Solution; Stable Peroxide Buffer; Sealing Tape; ELISA Wash Buffer (20X); Cell Lysis Buffer (10X)

Target Information
Target Name	Cyclic GMP
Target Description	Cyclic guanosine 3’, 5’-monophosphate (cGMP) is a critical and multifunctional second messenger molecule involved in many signal transduction pathways in different cell types of almost all species. Intracellular cGMP is generated from GTP by guanylyl cyclase (GC) and degraded through phosphodiesterase (PDE) hydrolysis. Two distinctive families of GC have been identified: soluble guanylyl cyclases (sGC) that are nitric oxide-responsive and cell membrane-bound; and particulate guanylyl cyclases (pGC) that respond to diverse extracellular agonists including peptide hormones, bacterial toxins, and free radicals. Phosphodiesterases form a superfamily of 11 isoforms with different specificity to both cyclic adenosine 3’, 5’-monophosphate (cAMP) and cGMP. Cyclic GMP regulates cellular physiology by activating cGMP-dependent kinase, modulating cGMP-dependent ion channels or transporters, and altering its own hydrolytic degradation by PDE. Because of the diversity of its effectors, cGMP plays an important role in regulating various pathological and physiological processes, such as vascular smooth muscle motility, intestinal fluid and electrolyte homeostasis, and retinal phototransduction.

Target Information

Target Name

Cyclic GMP

Target Description

Cyclic guanosine 3’, 5’-monophosphate (cGMP) is a critical and multifunctional second messenger molecule involved in many signal transduction pathways in different cell types of almost all species. Intracellular cGMP is generated from GTP by guanylyl cyclase (GC) and degraded through phosphodiesterase (PDE) hydrolysis. Two distinctive families of GC have been identified: soluble guanylyl cyclases (sGC) that are nitric oxide-responsive and cell membrane-bound; and particulate guanylyl cyclases (pGC) that respond to diverse extracellular agonists including peptide hormones, bacterial toxins, and free radicals. Phosphodiesterases form a superfamily of 11 isoforms with different specificity to both cyclic adenosine 3’, 5’-monophosphate (cAMP) and cGMP. Cyclic GMP regulates cellular physiology by activating cGMP-dependent kinase, modulating cGMP-dependent ion channels or transporters, and altering its own hydrolytic degradation by PDE. Because of the diversity of its effectors, cGMP plays an important role in regulating various pathological and physiological processes, such as vascular smooth muscle motility, intestinal fluid and electrolyte homeostasis, and retinal phototransduction.

Shipping & Storage
Sipping	Shipping on dry ice.
Storage	Store at -20°C. Please refer to protocols.

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