Cat. No.: DAA-001267
| Product Information | |
|---|---|
| Product Name | Active Rap1 Detection Kit |
| Description | The Active Rap1 Detection Kit provides all reagents necessary for measuring activation of Rap1 GTPase in the cell. GST-RalGDS-RBD fusion protein is used to bind the activated form of GTP-bound Rap1, which can then be immunoprecipitated with glutathione resin. Rap1 activation levels are then determined by western blot using a Rap1 Rabbit Antibody. |
| Component | GST-RalGDS-RBD; GDP; GTP γS; Rap1 Rabbit mAb; Glutathione Resin; SDS Sample Buffer; Lysis/Binding/Wash Buffer; Spin Cup and Collection Tubes |
| Target Information | |
|---|---|
| Target Name | RAP1A |
| UniProt No. | P62834 |
| Gene ID | 5906 |
| Target Description | The Ras superfamily of small GTP-binding proteins. These small G proteins have both GDP/GTP-binding and GTPase activities and function as binary switches in diverse cellular and developmental events that include cell cycle progression, cell survival, actin cytoskeletal organization, cell polarity and movement, and vesicular and nuclear transport. An upstream signal stimulates the dissociation of GDP from the GDP-bound form. The activated G protein then goes through a conformational change in its downstream effector-binding region, leading to the binding and regulation of downstream effectors. This activation can be switched off by the intrinsic GTPase activity, which hydrolyzes GTP to GDP and releases the downstream effectors. These intrinsic guanine nucleotide exchange and GTP hydrolysis activities of Ras superfamily proteins are also regulated by guanine nucleotide exchange factors. A wide variety of Rap GEFs have been identified: C3G connects Rap1 with RTKs through adaptor proteins such as Crk, Epacs. Rap1 and Rap2 are not fuctionally redundant as they perform overlapping but distinct functions. Recent research indicates that Rap2 regulates Dsh subcellular localization and is required for Wnt signaling in early development. |
| Alternative Names | C21KG G-22K; GTP-binding protein smg p21A; Ras-related protein Krev-1 |
| Shipping & Storage | |
|---|---|
| Sipping | Shipping on dry ice. |
| Storage | Store at -20°C. Please refer to protocols. |
