VA (Vitamin A) ELISA Kit
Online Inquiry
* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

VA (Vitamin A) ELISA Kit

Cat. No : CDEK-0372
size:
Target Information
Target Name VA (Vitamin A)
Synonyms VA
Product Properties
Prize Online Inquiry
Reactivity Universal
Assay Type Competitive-ELISA
Assay Time 2.5 h
Detection Range 15.63-1000 ng/mL
Sample Type Serum, plasma and other biological fluids
Sample Volume 50 μL
Sensitivity 9.38 ng/mL
Specificity This kit recognizes VA in samples. No significant cross-reactivity or interference between VA and analogues was observed.
Research Area Tags and Cell Markers, Cardiovascular, Metabolism
Reproducibility Both intra-CV and inter-CV are < 10%.
Precision Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level VA were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level VA were tested on 3 different plates, 20 replicates in each plate.
Storage -20 ℃. Please refer to the instructions for details.

Application

This ELISA kit applies to the in vitro quantitative determination of VA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VA. During the reaction, VA in the sample or standard competes with a fixed amount of VA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

! For research use only. Not intended for any clinical use.