TXA2 (Thromboxane A2) ELISA Kit
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* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

TXA2 (Thromboxane A2) ELISA Kit

Cat. No : CDEK-0462
size:
Target Information
Target Name TXA2 (Thromboxane A2)
Synonyms TXA2
Product Properties
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Reactivity Universal
Assay Type Competitive-ELISA
Assay Time 2.0 h
Detection Range 15.63-1000 pg/mL
Sample Type Serum, plasma and other biological fluids
Sample Volume 50 μL
Sensitivity 9.38 pg/mL
Specificity This kit recognizes TXA2 in samples. No significant cross-reactivity or interference between TXA2 and analogues was observed.
Research Area Cardiovascular, Metabolism, Signal Transduction, Cancer, Epigenetics and Nuclear Signal
Reproducibility Both intra-CV and inter-CV are < 10%.
Precision Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level TXA2 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level TXA2 were tested on 3 different plates, 20 replicates in each plate.
Storage -20 ℃. Please refer to the instructions for details.

Application

This ELISA kit applies to the in vitro quantitative determination of TXA2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with TXA2. During the reaction, TXA2 in the sample or standard competes with a fixed amount of TXA2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to TXA2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TXA2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

! For research use only. Not intended for any clinical use.