Recombinant Human Troponin C/TNNC1 Protein (His Tag)

Cat. No : CDRP-00753

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Target Information
Target Name	Troponin C/TNNC1
Synonyms	CMD1Z; CMH13; TN-C; TNC; TNNC
UniProt ID	P43025
Accession	P63316

Product Details
Expression Host	E.coli
Species	Human
Tag	N-His
Molar Mass	19.8 kDa
Sequence	Ser 26-Phe 131

Background
Troponin I, also known as TNI, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2(encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Background

Troponin I, also known as TNI, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2(encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Properties
Purity	>97% as determined by reducing SDS-PAGE.
Formulation	Supplied as a 0.2 μm filtered solution of 20 mM Tris, 100 mM NaCl, 1 mM DTT,10% Glycerol, pH8.0.
Reconstitution	Please refer to the printed manual for detailed information.
Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.
Storage	Store at < -20 °C, stable for 6 months. Please minimize freeze-thaw cycles.

! For research use only. Not intended for any clinical use.