Recombinant Human SMAD4 Protein (His Tag)
Online Inquiry
* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

Recombinant Human SMAD4 Protein (His Tag)

Cat. No : CDRP-00593
size:
Target Information
Target Name SMAD4
Synonyms Mothers Against Decapentaplegic Homolog 4; MAD Homolog 4; Mothers Against DPP Homolog 4; Deletion Target in Pancreatic Carcinoma 4; SMAD Family Member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4; MADH4
UniProt ID P22607
Accession Q13485
Product Details
Expression Host E.coli
Species Human
Tag C-6His
Molar Mass 61.5 kDa
Sequence Gln 24-Glu 524
Background
SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Properties
Purity >90% as determined by reducing SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, pH8.0.
Reconstitution Please refer to the printed manual for detailed information.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 °C. Reconstituted protein solution can be stored at 4-8 °C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 °C for 3 months.

! For research use only. Not intended for any clinical use.