Recombinant Human PFK2/PFKFB3 Protein (GST & His Tag)
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Recombinant Human PFK2/PFKFB3 Protein (GST & His Tag)

Cat. No : CDRP-00460
size:
Target Information
Target Name PFK2/PFKFB3
Synonyms IPFK2; PFK2; iPFK-2; PFK/FBPase 3
UniProt ID P13987
Accession Q16875-1
Product Details
Expression Host Baculovirus-Insect Cells
Species Human
Tag N-His & GST
Molar Mass 87.4 kDa
Sequence Tyr 95-Lys 267
Background
Fructose-2,6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, is a potent activator of phosphofructokinase, which is a rate-limiting enzyme of glycolysis. Highly phosphorylated PFKFB3 protein was found in human tumor cells, vascular endothelial cells, and smooth muscle cells. Fructose 2,6-bisphosphate (Fru-2,6-BP) is an allosteric activator of 6-phosphofructo-1-kinase (PFK-1), a rate-limiting enzyme and essential control point in glycolysis. The concentration of PFK2 depends on the activity of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase). PFK2 controls the glycolytic flux via the allosteric activator fructose 2,6-bisphosphate. Because of its proto-oncogenic character, the PFK-2/FBPase-2 of the PFKFB3 gene is assumed to play a critical role in tumorigenesis. The hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3) plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severe hypoxic conditions.
Properties
Purity >98% as determined by reducing SDS-PAGE.
Formulation Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH7.0.
Reconstitution Please refer to the printed manual for detailed information.
Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.
Storage Store at < -20 °C, stable for 6 months. Please minimize freeze-thaw cycles.

! For research use only. Not intended for any clinical use.