Products
Online Inquiry
Recombinant Human PFK2/PFKFB3 Protein (GST & His Tag)
Cat. No : CDRP-00460
size:
Target Information | |
---|---|
Target Name | PFK2/PFKFB3 |
Synonyms | IPFK2; PFK2; iPFK-2; PFK/FBPase 3 |
UniProt ID | P13987 |
Accession | Q16875-1 |
Product Details | |
---|---|
Expression Host | Baculovirus-Insect Cells |
Species | Human |
Tag | N-His & GST |
Molar Mass | 87.4 kDa |
Sequence | Tyr 95-Lys 267 |
Background |
---|
Fructose-2,6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, is a potent activator of phosphofructokinase, which is a rate-limiting enzyme of glycolysis. Highly phosphorylated PFKFB3 protein was found in human tumor cells, vascular endothelial cells, and smooth muscle cells. Fructose 2,6-bisphosphate (Fru-2,6-BP) is an allosteric activator of 6-phosphofructo-1-kinase (PFK-1), a rate-limiting enzyme and essential control point in glycolysis. The concentration of PFK2 depends on the activity of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase). PFK2 controls the glycolytic flux via the allosteric activator fructose 2,6-bisphosphate. Because of its proto-oncogenic character, the PFK-2/FBPase-2 of the PFKFB3 gene is assumed to play a critical role in tumorigenesis. The hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3) plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severe hypoxic conditions. |
Properties | |
---|---|
Purity | >98% as determined by reducing SDS-PAGE. |
Formulation | Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH7.0. |
Reconstitution | Please refer to the printed manual for detailed information. |
Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. |
Storage | Store at < -20 °C, stable for 6 months. Please minimize freeze-thaw cycles. |
! For research use only. Not intended for any clinical use.