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Recombinant Human PAPPA2/Pappalysin 2 Protein (His Tag)
Cat. No : CDRP-00427
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Target Information | |
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Target Name | PAPPA2/Pappalysin 2 |
Synonyms | PAPP-A2; PAPP-E; PAPPE; PLAC3 |
UniProt ID | P12034 |
Accession | NP_064714.2 |
Product Details | |
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Expression Host | HEK293 Cells |
Species | Human |
Tag | C-His |
Molar Mass | 131 kDa |
Sequence | Met 1-Pro 698 |
Background |
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Pappalysin-2/PAPP-A2 is the second member of the pappalysin family of metzincin superfamily, of which PAPP-A is the first member. There is no homology between the prepro-peptides of PAPP-A and PAPP-A2, but 46% of the residues of mature PAPP-A are also present in mature PAPP-A2. PAPP-A specifically cleaves insulin-like growth factor-binding protein(IGFBP)-4, one of six known modulators of IGF-I and –II, whereas PAPP-A2 specifically cleaved IGFBP-5 at one site, between Ser-143 and Lys-144. In contrast to the cleavage of IGFBP-4 by PAPP-A that strictly requires the presence of IGF, the cleavage of IGFBP-5 by PAPP-A2 was IGF-independent. Recent data firmly establish PAPP-A and IGFBP-4 as an important functional pair in several systems. Because of its close relationship with PAPP-A, both structurally and functionally, PAPP-A2 is a likely candidate for IGFBP-5 proteinase in many tissues and conditioned media where IGFBP-5 proteolysis has been reported. |
Properties | |
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Purity | >95% as determined by reducing SDS-PAGE. |
Formulation | Lyophilized from sterile PBS, pH7.4. |
Reconstitution | Please refer to the printed manual for detailed information. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 °C. Reconstituted protein solution can be stored at 4-8 °C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 °C for 3 months. |
! For research use only. Not intended for any clinical use.