Recombinant Human Osteonectin/SPARC Protein (His Tag)
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Recombinant Human Osteonectin/SPARC Protein (His Tag)

Cat. No : CDRP-00414
size:
Target Information
Target Name Osteonectin/SPARC
Synonyms SPARC; Basement-Membrane Protein 40; BM-40; Osteonectin; ON; Secreted Protein Acidic and Rich in Cysteine; SPARC; ON
UniProt ID P11247
Accession P09486
Product Details
Expression Host HEK293 Cells
Species Human
Tag C-6His
Molar Mass 33.7 kDa
Sequence Ala 279-Ser 390
Background
Secreted Protein Acidic and Rich in Cysteine (SPARC) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family. SPARC has 286 amino acids and contains an EF-hand in C-termina domain, a follistatin-like domain with Kazal-like sequences. There are two calcium binding sites, one binds 5 - 8 Ca2+ with a low affinity and other on an EF-hand loop that binds a Ca2+ ion with a high affinity. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC regulate cell growth through interactions with the extracellular matrix (ECM) and cytokines. SPARC bind to numerous proteins of the ECM, affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC also binds several types of collagen, albumin, thrombospondin, PDGF and cell membranes.
Properties
Purity >85% as determined by reducing SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution of 20 mM PBS, 150 mM NaCl, pH7.2.
Reconstitution Please refer to the printed manual for detailed information.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 °C. Reconstituted protein solution can be stored at 4-8 °C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 °C for 3 months.

! For research use only. Not intended for any clinical use.