Recombinant Human GRK5/GPRK5 Protein (His Tag)
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Recombinant Human GRK5/GPRK5 Protein (His Tag)

Cat. No : CDRP-00096
size:
Target Information
Target Name GRK5/GPRK5
Synonyms GPRK5
UniProt ID O95971
Accession NP_005299.1
Product Details
Expression Host Baculovirus-Insect Cells
Species Human
Tag C-His
Molar Mass 69 kDa
Sequence Met 1-Ala 248
Background
G protein-coupled receptor kinase 5, also known as G protein-coupled receptor kinase GRK5 and GRK5, is a member of the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRKs specifically phosphorylate agonist-occupied G protein-coupled receptors at the inner surface of the plasma membrane (PM), leading to receptor desensitization. GRKs utilize a variety of mechanisms to bind tightly, and sometimes reversibly, to cellular membranes. GRKs play an important role in mediating agonist-specific desensitization of numerous G protein-coupled receptors. GRK5 contains oneAGC-kinase C-terminal domain, oneprotein kinase domain and oneRGS domain. GRK5 specifically phosphorylates the activated forms of G protein-coupled receptors. Phospholipid-stimulated autophosphorylation may represent a novel mechanism for membrane association and regulation of GRK5 activity. GRK5 deficiency significantly exaggerates microgliosis and astrogliosis in the presence of an inflammatory initiator, such as the excess fibrillar Abeta and the subsequent active inflammatory reactions. GRK5 deficiency has been linked to early Alzheimer's disease in humans and mouse models of the disease.
Properties
Purity >95% as determined by reducing SDS-PAGE.
Formulation Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 1 mM DTT, 0.5 mM EDTA, 10% Glycerol, pH7.4.
Reconstitution Please refer to the printed manual for detailed information.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 °C. Reconstituted protein solution can be stored at 4-8 °C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 °C for 3 months.

! For research use only. Not intended for any clinical use.