Recombinant Human Calsequestrin-1/CASQ1 Protein
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Recombinant Human Calsequestrin-1/CASQ1 Protein

Cat. No : CDRP-00720
size:
Target Information
Target Name Calsequestrin-1/CASQ1
Synonyms CASQ; PDIB1; VMCQA
UniProt ID P36894
Accession P31415
Product Details
Expression Host E.coli
Species Human
Tag None
Molar Mass 41.8 kDa
Sequence Met 1-Ala 242
Background
Calsequestrin-1 is an isoform of calsequestrin. Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. It helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. Two forms of calsequestrin have been identified: Calsequestrin-2 and Calsequestrin-1. Calsequestrin-1 is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 50% of it adopting a random coil conformation. When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%. Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree. Calsequestrin-1 is also secreted in the gut where it deprives bacteria of calcium ions.
Properties
Purity >95% as determined by reducing SDS-PAGE.
Formulation Lyophilized from sterile 50 mM Tris, 10% Glycerol, pH7.5.
Reconstitution Please refer to the printed manual for detailed information.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 °C. Reconstituted protein solution can be stored at 4-8 °C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 °C for 3 months.

! For research use only. Not intended for any clinical use.