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Recombinant Human Calsequestrin-1/CASQ1 Protein
Cat. No : CDRP-00720
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Target Information | |
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Target Name | Calsequestrin-1/CASQ1 |
Synonyms | CASQ; PDIB1; VMCQA |
UniProt ID | P36894 |
Accession | P31415 |
Product Details | |
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Expression Host | E.coli |
Species | Human |
Tag | None |
Molar Mass | 41.8 kDa |
Sequence | Met 1-Ala 242 |
Background |
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Calsequestrin-1 is an isoform of calsequestrin. Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. It helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. Two forms of calsequestrin have been identified: Calsequestrin-2 and Calsequestrin-1. Calsequestrin-1 is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 50% of it adopting a random coil conformation. When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%. Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree. Calsequestrin-1 is also secreted in the gut where it deprives bacteria of calcium ions. |
Properties | |
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Purity | >95% as determined by reducing SDS-PAGE. |
Formulation | Lyophilized from sterile 50 mM Tris, 10% Glycerol, pH7.5. |
Reconstitution | Please refer to the printed manual for detailed information. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 °C. Reconstituted protein solution can be stored at 4-8 °C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 °C for 3 months. |
! For research use only. Not intended for any clinical use.