Recombinant Human ATG5 Protein

Cat. No : CDRP-01220

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Target Information
Target Name	ATG5
Synonyms	Autophagy protein 5; APG5-like; Apoptosis-specific protein; APG5L; ASP; ATG5
UniProt ID	Q9NPY3
Accession	Q9H1Y0

Product Details
Expression Host	E.coli
Species	Human
Tag	None
Molar Mass	32.4 kDa
Sequence	Ser 25-Ser 92

Background
ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.

Background

ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.

Properties
Purity	>97% as determined by reducing SDS-PAGE.
Formulation	Supplied as a 0.2 μm filtered solution of 20 mM Tris, 0.2M NaCl, 1 mM DTT, 40% Glycerol, pH8.0.
Reconstitution	Please refer to the printed manual for detailed information.
Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.
Storage	Store at < -20 °C, stable for 6 months. Please minimize freeze-thaw cycles.

! For research use only. Not intended for any clinical use.