Recombinant Human 5'-Nucleotidase/5'-NT/CD73 Protein (C-6His)

Cat. No : CDRP-00614

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Target Information
Target Name	5'-Nucleotidase/5'-NT/CD73
Synonyms	5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE
UniProt ID	P23680
Accession	P21589

Product Details
Expression Host	HEK293 Cells
Species	Human
Tag	C-6His
Molar Mass	58.8 kDa
Sequence	Ser 32-Thr 290

Background
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.

Background

CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.

Properties
Purity	>97% as determined by reducing SDS-PAGE.
Formulation	Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl₂, 20% Glycerol, pH7.5.
Reconstitution	Please refer to the printed manual for detailed information.
Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.
Storage	Store at < -20 °C, stable for 6 months. Please minimize freeze-thaw cycles.

! For research use only. Not intended for any clinical use.