Rat OPN (Osteopontin) ELISA Kit
| Target Information | |
|---|---|
| Target Name | OPN (Osteopontin) |
| Synonyms | SPP1, BNSP, BSP1, ETA-1, Bone Sialoprotein I, Secreted Phosphoprotein 1, Early T-Lymphocyte Activation 1 |
| Product Properties | |
|---|---|
| Prize | Online Inquiry |
| Reactivity | Rat |
| Assay Type | Sandwich-ELISA |
| Assay Time | 3.5 h |
| Detection Range | 1.56-100 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Sample Volume | 100 μL |
| Sensitivity | 0.94 ng/mL |
| Specificity | This kit recognizes Rat OPN in samples. No significant cross-reactivity or interference between Rat OPN and analogues was observed. |
| Research Area | Cancer, Cardiovascular, Signal Transduction, Stem Cells |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat OPN were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat OPN were tested on 3 different plates, 20 replicates in each plate. |
| Storage | -20 ℃. Please refer to the instructions for details. |
Application
This ELISA kit applies to the in vitro quantitative determination of Rat OPN concentrations in serum, plasma and other biological fluids.
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OPN. You can calculate the concentration of Rat OPN in the samples by comparing the OD of the samples to the standard curve.
! For research use only. Not intended for any clinical use.