Rat OPN (Osteopontin) ELISA Kit
Online Inquiry
* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

Rat OPN (Osteopontin) ELISA Kit

Cat. No : CDEK-0316
size:
Target Information
Target Name OPN (Osteopontin)
Synonyms SPP1, BNSP, BSP1, ETA-1, Bone Sialoprotein I, Secreted Phosphoprotein 1, Early T-Lymphocyte Activation 1
Product Properties
Prize Online Inquiry
Reactivity Rat
Assay Type Sandwich-ELISA
Assay Time 3.5 h
Detection Range 1.56-100 ng/mL
Sample Type Serum, plasma and other biological fluids
Sample Volume 100 μL
Sensitivity 0.94 ng/mL
Specificity This kit recognizes Rat OPN in samples. No significant cross-reactivity or interference between Rat OPN and analogues was observed.
Research Area Cancer, Cardiovascular, Signal Transduction, Stem Cells
Reproducibility Both intra-CV and inter-CV are < 10%.
Precision Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat OPN were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat OPN were tested on 3 different plates, 20 replicates in each plate.
Storage -20 ℃. Please refer to the instructions for details.

Application

This ELISA kit applies to the in vitro quantitative determination of Rat OPN concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OPN. You can calculate the concentration of Rat OPN in the samples by comparing the OD of the samples to the standard curve.

! For research use only. Not intended for any clinical use.