Rat HNF4a (Hepatocyte Nuclear Factor 4 Alpha) ELISA Kit
| Target Information | |
|---|---|
| Target Name | HNF4a (Hepatocyte Nuclear Factor 4 Alpha) |
| Product Properties | |
|---|---|
| Prize | Online Inquiry |
| Reactivity | Rat |
| Assay Type | Sandwich-ELISA |
| Assay Time | 3.5 h |
| Detection Range | 62.50-4000 pg/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Sample Volume | 100 μL |
| Sensitivity | 37.50 pg/mL |
| Specificity | This kit recognizes Rat HNF4a in samples. No significant cross-reactivity or interference between Rat HNF4a and analogues was observed. |
| Research Area | Cancer, Cardiovascular, Developmental Biology, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat HNF4a were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat HNF4a were tested on 3 different plates, 20 replicates in each plate. |
| Storage | -20 ℃. Please refer to the instructions for details. |
Application
This ELISA kit applies to the in vitro quantitative determination of Rat HNF4a concentrations in serum, plasma and other biological fluids.
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HNF4a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HNF4a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HNF4a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HNF4a. You can calculate the concentration of Rat HNF4a in the samples by comparing the OD of the samples to the standard curve.
! For research use only. Not intended for any clinical use.