Rat BNP (Brain Natriuretic Peptide) ELISA Kit
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* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

Rat BNP (Brain Natriuretic Peptide) ELISA Kit

Cat. No : CDEK-0577
size:
Target Information
Target Name BNP (Brain Natriuretic Peptide)
Synonyms NPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B
Product Properties
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Reactivity Rat
Assay Type Competitive-ELISA
Assay Time 2.0 h
Detection Range 31.25-2000 pg/mL
Sample Type Serum, plasma and other biological fluids
Sample Volume 50 μL
Sensitivity 18.75 pg/mL
Specificity This kit recognizes BNP in samples. No significant cross-reactivity or interference between BNP and analogues was observed.
Research Area Cancer, Cardiovascular, Neuroscience
Reproducibility Both intra-CV and inter-CV are < 10%.
Precision Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level BNP were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level BNP were tested on 3 different plates, 20 replicates in each plate.
Storage -20 ℃. Please refer to the instructions for details.

Application

This ELISA kit applies to the in vitro quantitative determination of BNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BNP. During the reaction, BNP in the sample or standard competes with a fixed amount of BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

! For research use only. Not intended for any clinical use.