QuicKey Human VEGF-A (Vascular Endothelial Growth Factor A) ELISA Kit
| Target Information | |
|---|---|
| Target Name | VEGF-A (Vascular Endothelial Growth Factor A) |
| UniProt ID | P15692 |
| Product Properties | |
|---|---|
| Prize | Online Inquiry |
| Reactivity | Human |
| Assay Type | Sandwich-ELISA |
| Assay Time | 2.5 h |
| Detection Range | 0.04-10 ng/mL |
| Sample Type | Serum and plasma |
| Sample Volume | 50 μL |
| Sensitivity | 0.03 ng/mL |
| Specificity | This kit recognizes Human VEGF-A in samples. No significant or interference between Human VEGF-A and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level human VEGF-A were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level human VEGF-A were tested on 3 different plates, 20 replicates in each plate. |
| Storage | -20 ℃. Please refer to the instructions for details. |
Application
This ELISA kit applies to the in vitro quantitative determination of Human VEGF-A concentrations in serum and plasma. Please consult technical support for the applicability if other biological fluids need to be tested.
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to human VEGF-A. Samples (or Standards) and biotinylated detection antibody specific for human VEGF-A are added to the micro ELISA plate wells. human VEGF-A would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain human VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of human VEGF-A. You can calculate the concentration of human VEGF-A in the samples by comparing the OD of the samples to the standard curve.
! For research use only. Not intended for any clinical use.