QuicKey Human TGF-α (Transforming Growth Factor Alpha) ELISA Kit
| Target Information | |
|---|---|
| Target Name | TGF-α (Transforming Growth Factor Alpha) |
| UniProt ID | P01135 |
| Product Properties | |
|---|---|
| Prize | Online Inquiry |
| Reactivity | Human |
| Assay Type | Sandwich-ELISA |
| Assay Time | 2.5 h |
| Detection Range | 31.25-2000 pg/mL |
| Sample Type | Serum and plasma |
| Sample Volume | 50 μL |
| Sensitivity | 7.54 pg/mL |
| Specificity | This kit recognizes Human TGF-α in samples. No significant or interference between Human TGF-α and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level human TGF-α were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level human TGF-α were tested on 3 different plates, 20 replicates in each plate. |
| Storage | -20 ℃. Please refer to the instructions for details. |
Application
This ELISA kit applies to the in vitro quantitative determination of Human TGF-α concentrations in serum and plasma. Please consult technical support for the applicability if other biological fluids need to be tested.
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to human TGF-α. Samples (or Standards) and biotinylated detection antibody specific for human TGF-α are added to the micro ELISA plate wells. human TGF-α would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain human TGF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of human TGF-α. You can calculate the concentration of human TGF-α in the samples by comparing the OD of the samples to the standard curve.
! For research use only. Not intended for any clinical use.