Mouse CRP (C-reactive Protein) ELISA Kit
| Target Information | |
|---|---|
| Target Name | CRP (C-reactive Protein) |
| UniProt ID | P14847 |
| Product Properties | |
|---|---|
| Prize | Online Inquiry |
| Reactivity | Mouse |
| Assay Type | Sandwich-ELISA |
| Assay Time | 3.5 h |
| Detection Range | 0.03-25 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Sample Volume | 100 μL |
| Sensitivity | 0.02 ng/mL |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Storage | 2-8 ℃ |
Application
Quantitative determination of Human CRP concentrations in human serum, plasma and other biological fluids.
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CRP. You can calculate the concentration of Mouse CRP in the samples by comparing the OD of the samples to the standard curve.
! For research use only. Not intended for any clinical use.