Mouse BNP (Brain Natriuretic Peptide) ELISA Kit
| Target Information | |
|---|---|
| Target Name | BNP (Brain Natriuretic Peptide) |
| UniProt ID | P40753 |
| Synonyms | NPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B |
| Product Properties | |
|---|---|
| Prize | Online Inquiry |
| Reactivity | Mouse |
| Assay Type | Competitive-ELISA |
| Assay Time | 2.0 h |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Sample Volume | 50 μL |
| Sensitivity | 9.38 pg/mL |
| Specificity | This kit recognizes BNP in samples. No significant cross-reactivity or interference between BNP and analogues was observed. |
| Research Area | Cancer, Cardiovascular, Neuroscience |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level BNP were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level BNP were tested on 3 different plates, 20 replicates in each plate. |
| Storage | -20 ℃. Please refer to the instructions for details. |
Application
This ELISA kit applies to the in vitro quantitative determination of BNP concentrations in serum, plasma and other biological fluids.
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BNP. During the reaction, BNP in the sample or standard competes with a fixed amount of BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
! For research use only. Not intended for any clinical use.