Mouse APLN (Apelin) ELISA Kit
| Target Information | |
|---|---|
| Target Name | APLN (Apelin) |
| UniProt ID | Q9R0R4 |
| Synonyms | APEL, XNPEP2 |
| Product Properties | |
|---|---|
| Prize | Online Inquiry |
| Reactivity | Mouse |
| Assay Type | Competitive-ELISA |
| Assay Time | 2.0 h |
| Detection Range | 125.00-8000 pg/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Sample Volume | 50 μL |
| Sensitivity | 75.00 pg/mL |
| Specificity | This kit recognizes APLN in samples. No significant cross-reactivity or interference between APLN and analogues was observed. |
| Research Area | Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level APLN were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level APLN were tested on 3 different plates, 20 replicates in each plate. |
| Storage | -20 ℃. Please refer to the instructions for details. |
Application
This ELISA kit applies to the in vitro quantitative determination of APLN concentrations in serum, plasma and other biological fluids.
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with APLN. During the reaction, APLN in the sample or standard competes with a fixed amount of APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to APLN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of APLN in tested samples can be calculated by comparing the OD of the samples to the standard curve.
! For research use only. Not intended for any clinical use.