Mouse Ang-II (Angiotensin II) ELISA Kit
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Mouse Ang-II (Angiotensin II) ELISA Kit

Cat. No : CDEK-0465
size:
Target Information
Target Name Ang-II (Angiotensin II)
UniProt ID P11859
Product Properties
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Reactivity Mouse
Assay Type Competitive-ELISA
Assay Time 2.0 h
Detection Range 15.63-1000 pg/mL
Sample Type Serum, plasma and other biological fluids
Sample Volume 50 μL
Sensitivity 9.38 pg/mL
Specificity This kit recognizes Ang-Ⅱ in samples. No significant cross-reactivity or interference between Ang-Ⅱ and analogues was observed.
Research Area Cancer, Cardiovascular, Metabolism, Signal Transduction
Reproducibility Both intra-CV and inter-CV are < 10%.
Precision Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Ang-Ⅱ were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Ang-Ⅱ were tested on 3 different plates, 20 replicates in each plate.
Storage -20 ℃. Please refer to the instructions for details.

Application

This ELISA kit applies to the in vitro quantitative determination of Ang-II concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang-Ⅱ. During the reaction, Ang-Ⅱ in the sample or standard competes with a fixed amount of Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.

! For research use only. Not intended for any clinical use.