Human PROS (Protein S) ELISA Kit
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* Please note that all of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

Human PROS (Protein S) ELISA Kit

Cat. No : CDEK-0733
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Target Information
Target Name PROS (Protein S)
UniProt ID P07225
Product Properties
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Reactivity Human
Assay Type Sandwich-ELISA
Assay Time 3.5 h
Detection Range 0.31-20 ng/mL
Sample Type Plasma
Sample Volume 100 μL
Sensitivity 0.19 ng/mL
Reproducibility Both intra-CV and inter-CV are < 10%.
Storage 2-8 ℃

Application

Quantitative determination of Human PROS concentrations in human plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to human PROS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for human PROS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain human PROS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of human PROS. You can calculate the concentration of human PROS in the samples by comparing the OD of the samples to the standard curve.

! For research use only. Not intended for any clinical use.