Human PCX (Podocalyxin) ELISA Kit
| Target Information | |
|---|---|
| Target Name | PCX (Podocalyxin) |
| UniProt ID | O00592 |
| Product Properties | |
|---|---|
| Prize | Online Inquiry |
| Reactivity | Human |
| Assay Type | Competitive-ELISA |
| Assay Time | 2.0 h |
| Detection Range | 0.16-10 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Sample Volume | 50 μL |
| Sensitivity | 0.10 ng/mL |
| Specificity | This kit recognizes PCX in samples. No significant cross-reactivity or interference between PCX and analogues was observed. |
| Research Area | Cancer, Cardiovascular, Signal Transduction, Stem Cells |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level PCX were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level PCX were tested on 3 different plates, 20 replicates in each plate. |
| Storage | -20 ℃. Please refer to the instructions for details. |
Application
This ELISA kit applies to the in vitro quantitative determination of PCX concentrations in serum, plasma and other biological fluids.
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PCX. During the reaction, PCX in the sample or standard competes with a fixed amount of PCX on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PCX. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PCX in tested samples can be calculated by comparing the OD of the samples to the standard curve.
! For research use only. Not intended for any clinical use.