Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit
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Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit

Cat. No : CDEK-0652
size:
Target Information
Target Name NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial)
UniProt ID P29474
Synonyms ECNOS, NOS III, E-NOS
Product Properties
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Reactivity Human
Assay Type Sandwich-ELISA
Assay Time 3.5 h
Detection Range 62.50-4000 pg/mL
Sample Type Serum, plasma and other biological fluids
Sample Volume 100 μL
Sensitivity 37.50 pg/mL
Specificity This kit recognizes Human NOS3/eNOS in samples. No significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed.
Research Area Cancer, Cardiovascular, Cell Biology, Metabolism, Neuroscience
Reproducibility Both intra-CV and inter-CV are < 10%.
Precision Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level human NOS3/eNOS were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level human NOS3/eNOS were tested on 3 different plates, 20 replicates in each plate.
Storage -20 ℃. Please refer to the instructions for details.

Application

This ELISA kit applies to the in vitro quantitative determination of Human NOS3/eNOS concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to human NOS3/eNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for human NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain human NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of human NOS3/eNOS. You can calculate the concentration of human NOS3/eNOS in the samples by comparing the OD of the samples to the standard curve.

! For research use only. Not intended for any clinical use.