Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit
| Target Information | |
|---|---|
| Target Name | HIF-1α (Hypoxia Inducible Factor 1 Alpha) |
| UniProt ID | Q16665 |
| Synonyms | HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor |
| Product Properties | |
|---|---|
| Prize | Online Inquiry |
| Reactivity | Human |
| Assay Type | Sandwich-ELISA |
| Assay Time | 3.5 h |
| Detection Range | 62.50-4000 pg/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Sample Volume | 100 μL |
| Sensitivity | 37.50 pg/mL |
| Specificity | This kit recognizes Human HIF-1α in samples. No significant cross-reactivity or interference between Human HIF-1α and analogues was observed. |
| Research Area | Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level human HIF-1α were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level human HIF-1α were tested on 3 different plates, 20 replicates in each plate. |
| Storage | -20 ℃. Please refer to the instructions for details. |
Application
This ELISA kit applies to the in vitro quantitative determination of Human HIF-1α concentrations in serum, plasma and other biological fluids.
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to human HIF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for human HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain human HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of human HIF-1α. You can calculate the concentration of human HIF-1α in the samples by comparing the OD of the samples to the standard curve.
! For research use only. Not intended for any clinical use.